Webb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … http://www.bioon.com.cn/reagent/show_product.asp?id=3900880
Plasmid Phis2 1 TaKaRa Bioz
Webb8 juli 2024 · pHis2 编号 载体名称 北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息 出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体 高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点,限制性内切酶 载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签 ... Webb12 apr. 2024 · 目的:筛选与启动子pHIS2-A互作的蛋白。. 方法:以启动子A构建到酵母单杂交启动子载体pHIS2,文库质粒为pGADT7-中山杉文库。. 材料:. 1.启动子克隆:pHIS2-A。. 根据实验需求进行扩增并酶切构建载体,两端的EcoRI和. SacI酶切位点,序列如下 ... citizens advice bureau lerwick shetland
酵母杂交实验常见问题——单杂知识及自激活检测篇_元件_-bait_作用
Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). http://www.coolaber.com/usr/2160/File/F/F4671.pdf WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … citizens advice bureau leongatha