Phic31 attp site

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WebAug 20, 2024 · Finally, the candidate Phic31 pseudo attP site located in chromosome 6 was identified as an optimal integration site compared to the pseudo attP site of chromosome … WebExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism Depositor Norbert Perrimon

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WebJan 14, 2024 · PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible … WebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for the strand exchange... proximus-torens

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WebOct 25, 2007 · It has previously been reported that pseudo-attP sites show some similarity to the native phiC31 attP site and that they share a common motif that contains a strong inverted repeat . The pseudosites observed in hESC were subjected to similar analysis, and we found that these sites shared a common motif with the phiC31 attP site ( Fig. 2A ). WebOct 8, 2024 · An engineered phiC31 “Disintegrase” able to make an attP site in Drosophila out of an attR-attL pair is described. This was used to generate attP sites at genomic locations where a mini-white (mini-w) transgene was subject to chromosomal position effects (CPE). The first step was random genomic integration of a P-element-based … WebWe injected the donor plasmid together with the phiC31 integrase mRNA into the embryos of the host strain and obtained positive lines. Structural analysis of the lines showed that site-specific integration occurred by recombination between the genomic attP site and the attB site of the donor plasmid. resting heart rate protocol

Chromosome mapping of attP landing sites in the strains for phiC31 …

Using phage integrases in a site-specific dual integrase cassette ...

WebOct 28, 2024 · This example illustrates a method of screening a variant of phiC31 integrase that recognizes a mutant attP site. The target mutant attP site was selected based on the following criteria: 1) it has a GC content of 20-60%; 2) it is symmetrically similar to the wildtype phiC31 attP site; 3) it is unique in the human genome; 4) it is intergenic; 5 ... WebFeb 20, 2013 · Introduction. phiC31 integrase is a site-specific recombinase, which is produced by a kind of bacteriophage (phiC31) in a strain of Streptomyces (1, 2).This enzyme is a member of serine recombinases that has an accurate and specific function to recombine DNA fragments between two identified sites, with approximately 30 base pairs … proximus towers immobelWebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … resting heart rate over 120

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Phic31 attp site

(PDF) Identification of a Specific Pseudo attP Site for Phage phiC3 …

WebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ... WebIt is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the …

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WebJan 2, 2024 · Specifically, the phiC31 integrase recognizes the attP sequence and introduces transgenes into this target site (Thorpe and Smith 1998 ). attP was introduced into the D. prolongata genome via piggyBac -mediated transgenesis and Kudo et al. ( 2024b) revealed the transformation efficiency and expression pattern in each strain with attP … WebThe phiC31 (ΦC31) integrase system allows for site-specific integration of transgenes. Further, many attP sites have been characterized for position effects. The bacteriophage …

WebSep 16, 2008 · In this method, which we term “site-specific integrase mediated repeated targeting” (SIRT), an attPattachment site for the phage phiC31 integrase is first targeted to the vicinity of the gene of interest by homologous recombination. WebPhiC31 Integrase Expression Plasmid Put the integrase into your PhiC31 and PinPoint genome editing projects with this vector that expresses PhiC31 Integrase for one-step single-copy transgenesis Non-viral transgene delivery Single-copy integration Preferential integration at active sites Delivery of inserts with no size constraints

WebThe Jump-In™ technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using PhiC31 integrase. Unlike the better-known recombinases such as Cre and Flp, PhiC31 integrase catalyzes recombination between two nonidentical sites and lacking a corresponding excision ... Webthrough recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, …

WebPhiC31 Integrase Mouse iPSC Reprogramming System With both iPSC-generating donor and PhiC31 Integrase Expression plasmids, this kit delivers a complete, non-viral integrase-based iPSC generation system Non-viral transgene delivery Single-copy integration Preferential integration at active sites Delivery of inserts with no size constraints

WebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, … proximus torhoutWebPhiC31 Integrase Expression Plasmid. Put the integrase into your PhiC31 and PinPoint genome editing projects with this vector that expresses PhiC31 Integrase for one-step … proximus torhout winkelWebApr 1, 2004 · Abstract. The φC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition sites. Here we show that this site-specific integration system also functions efficiently in Drosophila melanogaster in cultured cells and in … proximus torhout openingsurenWebJun 17, 2010 · Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. While the phiC31 system has been recently applied in mouse and human cell lines and in Drosophila, it has not been demonstrated whether it can also catalyze efficient DNA recombination in zebrafish. proximus travel surf worldWebJul 14, 2024 · The ΦC31 recombinase system is an important member of the S-rec family [ 2 ]. Both HK and ΦC31 systems comprise attB / attP attachment sites that serve as points of recombination, and the recombinases that catalyze recombination. In each family, DNA exchange requires host-encoded proteins for recombination that differ between systems. proximus trage wifiWebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … resting heart rate recoveryWebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. proximus travel passport top intense