Iq sybr green supermix bio rad
WebNov 25, 2011 · Quantitative Real-Time PCR (RT-qPCR) was carried out on iQ5 Cycler—Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA, ... in 96 well PCR plates, with 25 μL reaction mixture volume, using iQ SYBR ® Green Supermix (Bio-Rad), according to the manufacturer's instructions. The primers used for Real-Time expression … WebWith PowerUp SYBR Green Master Mix, we’ve taken the best of EXPRESS SYBR GreenER SuperMix and added additional capabilities for your gene expression analysis. EXPRESS SYBR GreenER qPCR SuperMix kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments.
Iq sybr green supermix bio rad
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WebJun 28, 2016 · RNA (2 µg) was used for first-strand cDNA synthesis with reverse transcriptase (Promega Corporation, Madison, WI, USA), according to the manufacturer's protocol. cDNA was amplified by qPCR in a CFX96 Touch Real-Time PCR Detection system with CFX Manager 3.0 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) using iQ … WebiQ™ SYBR ® Green Supermix, 100 x 50 µl rxns, 2.5 ml (2 x 1.25 ml) from Bio-Rad Be the first to write a review! Citations: (223) Use iQ SYBR ® Green Supermix with fluorescein in real-time PCR detection systems requiring a passive reference dye for well factor collection. Detect as few as 10 copies of template
WebAug 27, 2016 · iQ SYBR Green Supermix (BioRad) or similar. 3. iCycler IQ apparatus (Bio-Rad) or similar. 3 Methods. 3.1 Disinfection of Plant Material. 1. Disinfect externally the leaves with 70 % ethanol (v/v) for 2 min, 1 % sodium hypochlorite for 5 min, and rinse twice in sterile distilled water (see Note 1). 2. WebSYBR ® Green Supermix: iQ™ SYBR ® Grow Supermix EpiQ™ Chromatin: SYBR ® Green Supermix: SsoAdvanced Universal Probes Supermix: iTaq Universal Queries Supermix: iQ Supermix and iQ Multiple Powermix: iTaq™ Universal SYBR ® Green One-Step Equipment: iTaq Universal Acoustic One-Step Kit: Bio-Rad: CFX96™, CFX96 Touch™, CFX384 ...
WebApplied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes greatly simplify your experiment setup as they contain everything you need: buffer, dNTPs, thermostable hot-start DNA polymerase, and, of course, SYBR Green dye. WebThe proprietary buffer and stabilizers have been optimized exclusively for SYBR Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. This supermix provides the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts.
WebFeb 24, 2012 · The iTaq™ universal SYBR ® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument. iTaq universal supermixes let you: Obtain reliable and reproducible gene expression analysis results
WebPowerUp SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments.† It is also compatible with the Bio-Rad CFX96™, CFX384™, and iQ ™5 systems, as well as the Roche LightCycler 480 and Agilent Mx3005P™ systems. For optimal results, primer philip verwey farms hanford caWebMay 21, 2012 · Finally, we titered the SYBR Green I concentrations in standard PCR master mixes and compared the normalized expression of the genes icaA, bhp, aap, psmβ1 and agrB using 4 distinct biofilm forming S. epidermidis strains to the results obtained with commercially available kits. tryfan routeWebPerfeCTa SYBR ® Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). philip vicinoWebiTaq Universal SYBR Green Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl 2 , SYBR Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and ... philip vest chiefland floridaWebAug 10, 2011 · The iQ™ SYBR ® Green Supermix contains 3 mM MgCl 2. 5'-nuclease probe hydrolysis reactions consisted of 0.4 U iTaq DNA polymerase (BIO-RAD, Hercules, CA), 1 μL 10X iTaq™ buffer (BIO-RAD, Hercules, CA), 3 mM MgCl 2, 800 μM dNTPs, and 200 nM each of the forward and reverse primers and the probe, 20 to 2 × 10 6 copies of the synthetic … philip verwey farmsWebiQ™ SYBR® Green Supermix, 1,000 x 50 µl rxns, 25 ml (5 x 5 ml) by Bio-Rad. Manufacturer Bio-Rad Model: 1708886. ... See the iQ™ SYBR ® Green Supermix product page for … tryfan school bangorWebApr 20, 2010 · Bio-Rad offers the iQ SYBR Green Supermix which contains dNTPs, 50 U/ml iTaq DNA polymerase, 6 mM MgCl 2, SYBR Green I, 20 nM fluorescein. To this mixture, just add your primers and template DNA to start the reaction. tryfan school