Immortalized human podocyte cell line

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August undefined, 2024

WitrynaThe conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [9,74]. Podocytes were cultured in the medium which contained RPMI 1640 medium with 10% fetal bovine serum, and insulin–transferrin–selenium at 33 °C. For the differentiation, podocytes were … Witryna1 mar 2010 · The generation of podocytes cell lines from urine of patients and healthy volunteers is novel and will help to advance studies of podocyte cell biology, and further improvements in the approaches to cell transformation and/or cell culture techniques are needed to allow cultured podocytes to fully reproduce in vivo characteristics. …

Establishment of conditionally immortalized human glomerular

WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in … WitrynaThese results suggest that podocyte cell lines can be established consistently from human urine. The generation of podocyte cell lines from urine of patients and … imemories number

Cell culture of podocytes. Conditionally immortalized human podocytes ...

WitrynaA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol. 2002; 13: 630-638. Crossref; PubMed; Google Scholar, 20. Saleem M.A. Ni L. Witherden I. et al. Co-localization of nephrin, podocin, and the actin cytoskeleton: evidence for a role in podocyte foot process formation. WitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein levels and increased S1P levels. Witryna[Creative-bioarray] Immortalized Cell Lines 소개 > BRIC ... ... list of no starch breakfast

Cellosaurus cell line CIHP-1 (CVCL_W186)

Immortalized Primary Cells - Innoprot Stable Cell Lines

WitrynaConditionally immortalized human podocyte WT and CRISPR/CAS9 GLA KO cell lines were differentiated at 37 °C at 70% confluency for 10 to 14 days. Growth protocol Conditionally immortalized human podocytes were let to proliferate at 33°C in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS), Penicillin/Streptomycin, … Witryna18 kwi 2024 · The immortalized human podocyte cell line were purchased from Bristol University and cultured under their published protocol. Podocyte mRNA profiles of these cells were examined. Contributor(s) Yoshimura Y, Nishinakamura R: Citation(s) 30635375: Submission date: Jun 30, 2024: Last update date: Apr 18, 2024: imemoryblobstreamWitrynaCell Lines. HK2 cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium Low Glucose (DMEM LG) supplemented with 10% foetal bovine serum (FBS) (Euroclone S.p.A., Pero, MI, Italy). Immortalized human podocytes, previously characterized were cultured in DMEM High Glucose (Euroclone) with 10% FBS. Glomerular endothelial … imemories shipping address

"Witryna30 sie 2009 · podocyte markers in cultured urinary cells. Further study of urinary podocytes offers an open window to an understanding of glomerular disease. … " - Immortalized human podocyte cell line

Immortalized human podocyte cell line

[Creative-bioarray] Immortalized Cell Lines 소개 > BRIC

WitrynaCell line name: CIHP-1: Synonyms: Conditionally Immortalized Human Podocytes-1; AB8/13; AB 8/13; hiPOD; ciPOD: Accession: CVCL_W186: Resource Identification … WitrynaIn contrast, no significant change was observed in mesangial cells after 12 h, and even after 48 h the wounds were not completely closed. Until now, conditionally …

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WitrynaPlating of glomeruli onto a collagen surface is followed by an outgrowth of cobblestone-like cells that, after replating, differentiate into arborized, mature podocytes. Currently, the majority of research studies use immortalized podocytic cell lines most often derived from transgenic mice bearing a conditional immortalizing gene. Witryna17 maj 2012 · Conditionally immortalized human podocyte cell lines have been developed by transfection using both the temperature-sensitive mutant U19tsA58 of …

Witryna25 mar 2024 · conditionally immortalized cell line. 1 reference. described by source. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. 1 reference. A novel source of cultured podocytes. 1 reference. derived from organism type. Homo sapiens. Witryna18 sie 2024 · A conditionally immortalized murine podocyte cell line (E11) was obtained from Cell Line Services. Podocytes were propagated at 33 °C in RPMI1640 supplemented with 10% FBS and 10 U/mL of IFN-γ (Saint Louis, MO, USA) to enhance expression of thermosensitive T antigen. ... Shimada, H.; Rajagopalan, L.E. Rho …

Witryna14 lut 2015 · Cell culture and high glucose-induced podocytopathy: An immortalized mouse podocyte cell line 25 which had been generated by expression of a temperature-sensitive mutant of the SV40 Large-T ... Witryna17 wrz 2024 · Generally, conditionally immortalized podocytes have lost many of their phenotypes in vivo, including process formation at high cell density with conventional …

WitrynaHuman cell lines are a critical biological resource. These in vitro models of human biology are used in biomedical research, toxicology studies, bioindustry, drug …

WitrynaImmortalized Primary Cells. Innoprot offer Human and animal primary cells immortalized with SV40 antigen T and other immortalization techniques. All immortalized cell lines has been characterized for specific markers and other for functional assays. Showing 1–16 of 23 results. 1 2. list of norwich seasonsWitryna14 lut 2015 · Mouse podocytes immortalised by transduction with a temperature sensitive large T antigen SV40 construct as previously described 45, 46, were used … imemories ratingsWitrynaA breakthrough in cell biology research. Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology … imemories starter kitWitryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When cultivated at 37 °C, the SV40 T-antigen is inactivated for cell differentiation. The culture medium for human podocytes was RPMI 1640 Medium (Gibco, via Thermo Fisher … imemories trackingWitrynaThe podocyte is a key cell in the glomerulus in health and disease: until recently, human podocytes have been difficult to study in vitro. We have developed a conditionally immortalized human podocyte cell line transfected with a temperature-sensitive simian virus 40 transgene: when the transgene is inactivated in vitro, these … imemories usbWitrynaMouse Podocyte Cell Line Mpc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... imemories website log inWitryna1 kwi 2002 · The conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [8, 40]. Podocytes … imemories telephone number