WebAug 28, 2024 · The Ctcf floxed allele and Nkx2.5-Cre line have been previously described [17, 23]. Primers used for genotyping are detailed in S8 Table. Ctcf fl/+ or Ctcf fl/fl … WebFeb 20, 2024 · The postdoctoral researchers Dr. Stanimir Dulev and Dr. Yang Li confirmed interaction and co-localisation of SA and CTCF in the absence of cohesin using siRNA …
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WebJun 15, 2005 · Abstract. CTCF is a candidate tumor suppressor gene encoding a multifunctional transcription factor. Surprisingly for a tumor suppressor, the levels of CTCF in breast cancer cell lines and tumors were found elevated compared with breast cell lines with finite life span and normal breast tissues. In this study, we aimed to investigate the … WebMar 15, 2012 · The genome of higher eukaryotes exhibits a patchwork of inactive and active genes. The nuclear protein CCCTC-binding factor (CTCF) when bound to insulator sequences can prevent undesirable crosstalk between active and inactive genomic regions, and it can also shield particular genes from enhancer function, a role that has many … how do they make boneless wings
Acute depletion of CTCF directly affects - Oxford Academic
WebJun 28, 2024 · These CTCF–CTCF interactions mostly initiate chromatin loops when the CTCF-bound sites are in a convergent (i.e. forward and reverse) orientation (23, 24) … Webgenome organization, with loss of CTCF leading dose depen-dently to insulation defects at most TAD boundaries and abro-gating the accumulation of chromatin loops between … WebDec 13, 2024 · Our results are consistent with cohesin-mediated loop extrusion in cells, and with CTCF both stopping and stabilizing cohesin. Surprisingly, the Fbn2 loop is both rare and dynamic, with a looped ... how do they make brass cartridges